> Hello trinity users
> I used Trinity to assemble ~700M paired-end reads using the following
> command
> "Trinity.pl --seqType fq --left left.fq --right right.fq --SS_lib_type FR
> --output ./ --JM 300G --CPU 15"
> The run was successful, and I got a list of contigs.
> Now I realize that the data was not strand specific, and I am wondering
> how
> does the adding of "--SS_lib_type FR" parameter affect the assembly.
> Shall I redo the assembly?
> Thanks
> Mali
>

I don't think this is the mailing list you are looking for.  Perhaps you
meant the Trinity RNA sequencer list at
https://lists.sourceforge.net/lists/listinfo/trinityrnaseq-users ?

This list is for discussion about the Trinity Desktop Environment:
http://www.trinitydesktop.org/

Tim